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• Follow the manufacturer's instructions for safe. Behind the scenes at the laboratory Inspire article. Staff are also skilled in providing the technical services researchers need, including performing microinjection procedures to create transgenic flies, carrying out genetic crosses, and setting up fly cages to run experiments – as well as undertaking the delicate task of identifying the sex of individual flies. Lab scenes in movies. A regulator or step-down pressure valve should be used to pressurize low-pressure equipment from a high-pressure source. Distillation or similar operations requiring a vacuum must use a trapping device to protect the vacuum source, personnel, and the environment. Chemical splash goggles have splash-proof sides to fully protect the eyes. If the back of the vacuum setup faces the open laboratory, protect it with panels of suitably heavy transparent plastic to prevent injury to nearby personnel from flying glass in case of implosion.

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Extra precautions are particularly important for instruments that incorporate high-voltage circuitry. Never use them in closed systems, where they may develop uncontrolled and dangerously high pressures. For instance, operations involving a chemical hazard and sharp objects may require the combined use of a chemical-resistant glove and a cut-resistant glove. Special care is needed when installing and placing water lines (used, for example, to cool equipment such as flash lamps for lasers) so that they do not leak or produce condensation, which can dampen power cables nearby. The use of water aspirators is discouraged. Metal-to-metal seals are machined to tolerances that seal without the need of Teflon tape or other gasketing materials. Model 2 scenes in the lab answers. For individuals with. Plastic locking disconnects can make it easy to unfasten water lines without having to unclamp and reclamp secured lines. In addition, the potential for a flood is greatly increased. When used on cylinders of flammable, toxic, or otherwise hazardous gases, vent the relief valve to a laboratory chemical hood or other safe location.

• Wear insulating rubber gloves when working with electrical equipment. Ment are carried out, the devices must be deenergized and all capacitors discharged safely. Experiments in supercritical fluids include high pressure and should be carried out with appropriate protective systems. Model 2 scenes in the lab answer. 1% for higher quality gauges. • Orient pressure-relief devices with the vent side of the device directed away from the operator or.

However, hydrostatic proof tests are necessary for final acceptance. Do not use water directly on fires that involve live electrical equipment (Class C fires) or chemicals such as alkali metals, metal hydrides, and metal alkyls that react vigorously with water (Class D fires). Personal clothing should fully cover the body. Electronic monitors and alarms are available to prevent hazards due to asphyxiant, flammable, and many toxic gases. These kits are used to confine and limit the spill if such actions can be taken without risk of injury or contamination. Before beginning any work, all personnel should be shown and trained in the use of all electrical power sources and the location of emergency shutoff switches. If the possibility exists that the temperature outside of the cylinder may increase to greater than 30°C, a lower percentage (e. g., 60%) of capacity should be the limit. 3) and personal safety techniques (section 7. Many electrical instruments, such as lasers and X-ray, electron-beam, radioactive, photochemical, and electrophoresis equipment, emit potentially harmful radiation, and, therefore, special precautions must be taken when they are used. Information regarding the vessel's history should include temperature extremes it has experienced, any modifications and repairs made to the original vessel, and all inspections or test actions it has undergone. Eyewash units are required in research or instructional laboratories if substances used there present an eye hazard or if unknown hazards may be encountered. Thus, the atmosphere in a refrigerator could contain an explosive mixture of air and the vapor of a flammable substance or a dangerously high concentration of the vapor of a toxic substance or both.

Normally, these two spark sources are located in the lower part of the hot plate in a region where any heavier-than-air and possibly flammable vapors evolving from a boiling liquid on the hot plate would tend to accumulate. Secure compressed gas cylinders firmly at all times. Improper loading and balancing can cause the rotors to dislodge while spinning. Solid desiccants are preferred. D. none of the abovecContact lenses 1. should/shouldn't be notHow should you dress your hair properly? Chemical splash goggles that conform to ANSI standard Z87. The fire was extinguished immediately but continued to flare up because the oil was still above its autoignition temperature. Corrosive or otherwise hazardous service requires more frequent tests and inspections. Trained laboratory personnel are also encouraged to wear long sleeves when handling cryogenic fluids.

The U. S. Department of Transportation (DOT) has established codes that specify the materials to be used for the construction and the capacities, test procedures, and service pressures of the cylinders in which compressed gases are transported. Then, when the liquid nitrogen bath is removed or when it evaporates, the condensed. In many cases, safety shoes are advisable. "But when you see the work they're doing, you feel part of a whole.

1 for additional information. Do not allow water, solvents, and corrosive gases to be drawn into a building vacuum system. Such conditions can push liquids into unwanted locations, sometimes with undesirable consequences. Tubing and piping downstream of such devices must be at least the same diameter as the fitting on the vent side of the relief device. Use them for immediate first-aid treatment of chemical splashes and for extinguishing clothing fires.

Use laboratory heating devices with a variable autotransformer to control and limit the input voltage to some fraction of the total line voltage, typically 110 V. If a variable autotransformer is not wired in this manner, the switch on it may or may not disconnect both wires of the output from the 110-V line when it is switched to the off position. If used where not needed, as on CGA fittings, Teflon tape only spreads and weakens the threaded connections and can plug up lines that it enters accidentally. Leave the valve protection cap in place until the cylinder is secured and ready for use. The following reminders are included for qualified personnel: • Always assume that a voltage potential exists within a device while servicing it, even if it is deenergized and disconnected from its power source. These devices differ in operation and purpose from fuses and circuit breakers, which are designed primarily to protect equipment and prevent electrical fires due to short circuits or other abnormally high current draw situations. The proper choice of glassware or metal tubes and joints is required, and the procedures should conform to safe practice with electrical equipment and evacuated apparatus. If the attachment fails, the impeller shaft could fall through the bottom of a glass vessel below, risking flying glass and a spill. Only qualified personnel wearing proper personal protective equipment (PPE) are allowed to open these boxes to access the main switches and circuit breakers contained therein. Because the unused chemicals may have reacted to something... and the substance may have changed entirelyWhat should you use to fill a pipette? Do not exceed the manufacturer's loading limits. In a biomedical laboratory, cells are the living systems that many scientists use as biological models for their experiments. Human exposure to ultrasound with frequencies of between 16 and 100 kHz can be divided into three distinct categories: airborne conduction, direct contact through a liquid-coupling medium, and direct contact with a vibrating solid.

The flying shrapnel damaged a refrigerator and freezer and gouged holes in the walls and ceiling. Regular inspection of their serviceability is important. Trained laboratory personnel are strongly advised to consult an expert on high-pressure work as they design, build, and operate a high-pressure process. Areas where large amounts of flammable solvents are in use also require explosion-proof lighting and electrical fixtures. 3 Automatic External Defbrillators (AED). Minimize glass cuts by use of correct procedures (e. g., the procedure for inserting glass tubing into rubber stoppers and tubing, which is taught in introductory laboratories), through appropriate use of protective equipment, and by careful attention to manipulation. 4. at the handles onlyIf you have a medical condition, who should you talk to before working in the lab?

Use only spark-free induction motors in power stirring and mixing devices or any other rotating equipment used for laboratory operations. Their use in filtration or solvent-removal operations involving volatile organic solvents presents a hazard that volatile chemicals will contaminate the wastewater and the sewer, even if traps are in place. If the motion of the impeller is impeded, the rubber can twist away from the motor shaft, and the motor will not stall. Modify any existing instrument that lacks these features to incorporate them. • Equip motor-driven electrical equipment used in a laboratory where volatile flammable materials may be present with either nonsparking induction motors that meet Class 1, Division 2, Group C-D electrical standards (Earley, 2008; NFPA, 2008) or air motors instead of series-wound motors that use carbon brushes, such as those generally used in vacuum pumps, mechanical shakers, stirring motors, magnetic stirrers, and rotary evaporators. "Code one, code one"What should you do if a chemical splashes in your eyes? Pressure-test and leak-test final assemblies to ensure their integrity. This allows for rapid removal of heat in the case of overheating or exothermicity. Do not use ovens to dry any chemical sample that has even moderate volatility and might pose a hazard.

An eyewash unit provides a soft stream or spray of aerated water for an extended period (15 minutes). • Inspect and replace pressure-relief devices periodically. Caps provide a vapor-tight seal to prevent a spill if the container is tipped over. Never leave the cylinder valve open when the equipment is not in use. Reject parts having damaged or partly stripped threads (see also section 7. AED owners should be familiar with local laws concerning training and use of these devices. Attention must be paid to both the mechanical and the electrical hazards inherent in using these devices. Mount pressure gauges so that they are easily read during operation. However, never use oil or lubricant on any equipment that will be used with oxygen.

Tailor them to deal with the potential risk associated with the materials being used in the laboratory. The plugCan hands be wet (but only very little) when touching electrical equipment? Thus, you must always l-------e glassware before putting it in the stopper. • Inform each individual working with electrical equipment of basic precautionary steps to take to ensure personal safety. For those solvents that are incompatible with copper (e. g., tetrahydrofuran, methylene chloride, acetonitrile), a second column of alumnia is used along with a dry nitrogen or argon purge to facilitate oxygen removal. Although nitrogen is inert, its liquefied form can be hazardous because of its cryogenic properties and because displacement of air oxygen in the vicinity can lead to asphyxiation followed by death with little warning. If use of an extension cord is necessary, use only a three-wire cord with a rating equal to or greater than that for the oven. Laboratory heat guns are constructed with a motor-driven fan that blows air over an electrically heated filament. Laboratory personnel should be certain that all electrical equipment is well maintained, properly located, and safely used. Resistance devices used to heat oil baths should not contain bare wires.

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